Determination of piceid in commercial Rhizoma Polygoni Cuspidati by HPLC.
- Author:
Lei WANG
1
;
Lan HUANG
;
Mian ZHANG
;
Zheng-tao WANG
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; analysis; Glucosides; analysis; Plant Roots; chemistry; Plants, Medicinal; chemistry; Polygonum; chemistry; Quality Control; Rhizome; chemistry; Species Specificity; Stilbenes; analysis
- From: China Journal of Chinese Materia Medica 2002;27(5):344-347
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo improve a RP-HPLC method of determining piceid in commercial Rhizoma Polygoni Cuspidati.
METHODHPLC method was used, with C18 column (250 mm x 4.6 mm), a mixture of CH3CN-H2O (20:80) as the mobile phase, and detecting wavelength 303 nm.
RESULTThe linear range was 0.0066-0.0792 microgram.microL-1, r = 0.9999 (n = 5). The average recovery rate was 98.5% and RSD was 2.5% (n = 9).
CONCLUSIONThe method is simple, accurate and reproducible. The contents of piceid in commercial Rhizoma Polygoni Cuspidati from different areas are variable and the average content is 2.23%.
