HPLC determination of 6-gingerol in Rhizoma Zingiberis Recens.
- Author:
Wei-hao WANG
1
;
Zhi-min WANG
;
Li-zhen XU
;
Shi-lin YANG
Author Information
- Publication Type:Journal Article
- MeSH: Catechols; Chromatography, High Pressure Liquid; Fatty Alcohols; analysis; Ginger; chemistry; Plants, Medicinal; chemistry; Quality Control; Rhizome; chemistry
- From: China Journal of Chinese Materia Medica 2002;27(5):348-349
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the contents of 6-Gingerol in Rhizoma Zingiberis Recens.
METHODHPLC method was used, with Alltech C18 column, acetonitrile-methol-water (43:5:52) as mobile phase with a flow rate of 0.8 mL.min-1, detecting wavelength 280 nm, and column temperature 35 degrees C.
RESULTRetained time of 6-gingerol was near 19 min, showing a good recovery (98.2%) and linear correlation (r = 0.9999). The contents of 6-gingerol were 1.35-2.87 mg.g-1, and the water contents were 70.4-85.5% mL.g-1 in Rhizoma Zingiberis Recens.
CONCLUSIONThe method is appropriate for the determination of 6-gingerol in Rhizoma Zingiberis Recens. Gingerol can be used as a chemical marker of the quality control of Rhizoma Zingiberis Recens.
