Study on stem-tip tissue culture of the traditional Chinese medicine Chrysanthemum morifolium.
- Author:
Jian-ping XUE
1
;
Ai-min ZHANG
;
Wei SHENG
;
Feng-lan ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Chrysanthemum; growth & development; Culture Techniques; Plant Stems; growth & development
- From: China Journal of Chinese Materia Medica 2002;27(5):350-360
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo set up the optimums for the stem-tip tissue culture of Chrysanthemum morifolium cultivated in Anhui Province.
METHODSmall sections (about 0.5 mm in length) from the stem-tips were isolated and inoculated with different media, and induced to form the whole plantlet formation.
RESULT AND CONCLUSIONThe MS medium added with 6-BA 2 mg.L-1 + NAA 0.2 mg.L-1 was the optimum medium for the bud sprouting and the inducing rate was over 80% after 40 d cultivation on this modified medium. The MS medium supplemented with 6-BA 2 mg.L-1 and NAA 0.5 mg.L-1 was the optimum medium for the multiplication of the adventitious buds in which the bud multiplication was about 4-7 times higher after 25-30 d cultivation. The plantlet could root well on the MS medium with NAA 0.5 mg.L-1.
