Screening of antimitotic portions from sea hare using conidia of Pyricularia Oryzae.
- Author:
Hou-wen LIN
1
;
Hai-feng TANG
;
Chun ZHANG
;
Yang SHEN
;
Cheng GUO
;
Yang-hua YI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antineoplastic Agents; isolation & purification; pharmacology; Aplysia; chemistry; HL-60 Cells; drug effects; Humans; Leukemia P388; pathology; Materia Medica; isolation & purification; pharmacology; Mice; Mitosis; drug effects; Mitosporic Fungi; physiology; Tumor Cells, Cultured; drug effects
- From: China Journal of Chinese Materia Medica 2002;27(1):53-55
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo apply conidia of Pyricularia Oryzae to the screening of antimitotic constituents from marine animal sea hare.
METHODTo extract and fractionate active portions from sea hare through detecting deformation of mycelia germinated from conidia of P. Oryzae P-2b, in comparison with the cytotoxic test results in vitro.
RESULTTwo active portions, of which IC50 against P388 and HL-60 was 23.4, 18.6 and 19.4, 12.5 micrograms.ml-1, respectively, were screened from this animal.
CONCLUSIONThis bioassay method was efficiently applied to the primary screening of antimitotic portions from marine animals for the first time. Being convenient, speedy and cheap, the screening model is suitable for the bioassay of active constituents from marine life.
