Measurement of baicalin contents by HPLC.
- Author:
Zhu-ying HE
1
;
Pei-xue CAO
;
Guang-yi LIANG
;
Zai-chuan LIU
;
Xing-guo WU
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; Flavonoids; analysis; Hot Temperature; Plants, Medicinal; chemistry; Scutellaria baicalensis; chemistry; Technology, Pharmaceutical
- From: China Journal of Chinese Materia Medica 2002;27(4):258-260
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare the contents of baicalin in the raw medical material and the different prepared medical materials of Scutellaria baicalensis.
METHODThe contents of baicalin were determined by HPLC. Chromatographic conditions included Hypersil C18 column and the mobile phase consisting of a mixture of methanol-0.04% phosphoric acid (46:54). Baicalin was detected at 280 nm. The standard curve was linear in the range of 0.0280-4.6700 micrograms with correlation coefficient 1.0000. The average recovery of baicalin was 101.22% with RSD = 2.54% (n = 5).
RESULTThe contents of baicalin were 6.80% in raw material, 6.00% and 6.73% in the different prepared medical materials of S. baicalensis respectively.
CONCLUSIONThe two different kinds of preparing methods can be used to the raw material of S. baicalensis.
