Isolation, cultivation and identification of neural stem cell from human embryonic CNS.
- Author:
Lan WANG
1
;
Huozhen HU
;
Chenghu ZHANG
;
Xiaoyu LI
;
Dachang TAO
;
Fen CHEN
Author Information
1. Cell Biology Department, West China Medical Center, Sicuan University, Chengdu 610041.
- Publication Type:Journal Article
- MeSH:
Astrocytes;
cytology;
Brain;
cytology;
embryology;
Cell Differentiation;
Cells, Cultured;
Culture Media;
chemistry;
Epidermal Growth Factor;
chemistry;
Fibroblast Growth Factor 2;
chemistry;
Humans;
Immunohistochemistry;
Neural Stem Cells;
cytology;
Neurons;
cytology;
Oligodendroglia;
Pluripotent Stem Cells;
cytology
- From:
Journal of Biomedical Engineering
2002;19(2):264-267
- CountryChina
- Language:Chinese
-
Abstract:
This is a study on the cultivation condition in vitro and differentiation of neural stem cells from human embryonic brain in order to find a way to get purified multipotential neural stem cells. The single cells was derived from the three-month embryonic brain digested with trypsin, some cells was frozen, the other cells were expanded with EGF and bFGF, the single-cell-clone was obtained by the way of limited dilution, and the serum was used to induce the cells differentiation. The cells were detected with the method of immunohistochemistry. The results showed that a lot of neurospheres could be seen in the presence of mitogens (both EGF and bFGF) and serum could induce neural stem cells to differentiate into neurons, astrocytes, and oligodendrocytes. These indicate that the survival and proliferation of neural stem cells rely on the cooperation of EGF and bFGF. The neural stem cells can also be harvested from the frozen cells.
