Development of conductance measurement technique for permeability of monolayer endothelial cells.
- Author:
Lei CHEN
1
;
Jianmin WANG
;
Jicheng JIN
Author Information
1. Institute of Surgery Research, Daping Hospital of the Third Military Medical University, Chongqing 400042. Key Lab of Biomechanics & Tissue Engineering under the State Ministry of Education, Chongqing University, Chongqing 400044.
- Publication Type:Journal Article
- MeSH:
Cell Culture Techniques;
Cells, Cultured;
Endothelial Cells;
cytology;
Humans;
Permeability
- From:
Journal of Biomedical Engineering
2002;19(2):273-283
- CountryChina
- Language:Chinese
-
Abstract:
The conductance measurement method of endothelial permeability has been established by the principle that there is direct ratio between conductance and filter area, and has been compared with the albumin filter method. The results show that there is close correlation between conductance and filter area, the conductance measurement can be used for the detection of the monolayer endothelial cells permeability. The combination of the conductance measurement and the albumin filter measurement method can accurately reflect the permeability change in severity and scope.
