Cloning of AE1-c-end cDNA and construction of its expression plasmid for yeast two-hybrid system.
- Author:
Hongtao LI
1
;
Guohui FU
;
Yong QIN
;
Hongqing DU
;
Xiaoshu JIANG
;
Ming LIU
;
Xiangang KONG
Author Information
1. Department of Pathophysiology, Harbin Medical University, Harbin 150086.
- Publication Type:Journal Article
- MeSH:
Cloning, Molecular;
DNA, Complementary;
Plasmids;
Polymerase Chain Reaction;
Two-Hybrid System Techniques;
Yeasts
- From:
Journal of Biomedical Engineering
2002;19(2):284-290
- CountryChina
- Language:Chinese
-
Abstract:
In this study, about 350 bp cDNA fragment was amplified by PCR. After being sequenced, the AE1-c-end gene fragment was cloned into EcoR I-Pst I site of pGADT7 to form AD ends in the yeast two-hybrid system. The recombinant plasmid was transformed into yeast AH109, and the expression in the yeast was observed. The results demonstrate that AE1-c-end was obtained. pGADT7-AE1-c-end has no toxic effect on the yeast. It can serve as a target gene of yeast two-hybrid system.
