Preliminary molecular analysis of bacterial composition in periapical lesions with primary endodontic infections of deciduous teeth.
- Author:
Jia-jian SHANG
1
;
Qiu-bo YANG
;
Huan-ying ZHAO
;
Shuang CAI
;
Yan ZHOU
;
Zheng SUN
Author Information
- Publication Type:Journal Article
- MeSH: Bacteria; classification; genetics; isolation & purification; Bacterial Infections; microbiology; Child; Child, Preschool; Female; Humans; Male; Periapical Tissue; microbiology; RNA, Ribosomal, 16S; genetics; Tooth, Deciduous; microbiology
- From: Chinese Medical Journal 2013;126(16):3112-3117
- CountryChina
- Language:English
-
Abstract:
BACKGROUNDThe bacterial composition of periapical lesions in deciduous teeth has not been well documented. This study was designed to explore the bacterial compositions, especially the dominant bacteria in periapical lesions using 16S rRNA sequencing.
METHODSTissue samples were collected from 11 periapical lesions in deciduous teeth with primary endodontic infections. DNA was extracted from each sample and analyzed using 16S rRNA cloning and sequencing for the identification of bacteria.
RESULTSAll DNA samples were positive for 16S rRNA gene PCR. One hundred and fifty-one phylotypes from 810 clones were identified to eight phyla, and each sample contained an average of 25.9 phylotypes. In addition, 59 phylotypes were detected in more than two samples, and Fusobacterium (F.) nucleatum (8/11), Dialister (D.) invisus (8/11), Campylobacter (C.) gracilis (7/11), Escherichia (E.) coli DH1 (6/11), Aggregatibacter (A.) segnis (6/11), and Streptococcus (S.) mitis (6/11) were the most prevalent species. Furthermore, 45 as-yet-uncultivated phylotypes were also identified.
CONCLUSIONSChronic periapical lesions in deciduous teeth contained polymicrobial infections. F. nucleatum, D. invisus, C. gracilis, E. coli DH1, A. segnis, and S. mitis were the most prevalent species detected by 16S rRNA sequencing.
