The study of hBMP-4 gene modified tissue-engineered bone.
- Author:
Xin-quan JIANG
1
;
Zhi-yuan ZHANG
;
Jian-guo CHEN
;
Gui-xiang MA
;
Chuan-jun CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bone Morphogenetic Protein 4; Bone Morphogenetic Proteins; genetics; Genetic Therapy; Humans; Mice; Mice, Nude; Osteogenesis; Rabbits; Tissue Engineering
- From:Chinese Journal of Stomatology 2003;38(5):390-392
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEBone marrow stromal cells (bMSCs) of rabbits transferred with mammalian hBMP-4 expression plasmid were used to construct tissue-engineered bone. Gene therapy combined with tissue-engineering technique was explored to further improve osteogenesis.
METHODSpEGFP-hBMP-4 plasmid was constructed by subcloning technique. bMSCs were then transferred with either pEGFP-hBMP-4, pEGFP plasmid by lipofectamine or left uninfected in vitro. The cells from the 3 groups were combined with natural non-organic bone (NNB) to construct tissue-engineered bones, which were subcutaneously implanted into nude mice (6 implants per group) for 4 weeks. Specimens were evaluated through histological and computerized new bone formation analysis.
RESULTSpEGFP-hBMP-4 plasmid was successfully constructed. bMSCs could attach and proliferate on the surface on NNB. In vivo experiment showed that new bone formation in pEGFP-hBMP-4 group was higher than those of the control groups.
CONCLUSIONSTissue-engineered bone using hBMP-4 gene modified bMSCs might be an ideal alternative for the repair of bone.
