Bioassay of the soluble human tumor necrosis factor receptor I recombinant plasmid expression in vitro.
- Author:
Chen-rong XU
1
;
Jin-cai ZHANG
;
Chuan-jiang ZHAO
;
Yun-hui ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antigens, Surface; biosynthesis; CHO Cells; Cloning, Molecular; Cricetinae; DNA, Complementary; biosynthesis; genetics; Eukaryotic Cells; metabolism; Genetic Vectors; drug effects; Humans; Plasmids; biosynthesis; genetics; Receptors, Tumor Necrosis Factor, Type I; biosynthesis; genetics; Recombinant Fusion Proteins; biosynthesis; genetics; pharmacology; Transfection
- From: Chinese Journal of Stomatology 2004;39(3):185-188
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect the expression of recombinant plasmid PcDNA3.1-sTNFRI in vitro and evaluate the bioactivity of expressed sTNFRI.
METHODSCHO cells were transfected with recombinant plasmid PcDNA3.1-sTNFRI by liposome. sTNFRI in cell culture supernatant was detected by ELISA and sTNFRI blockage of TNF-alpha cytotoxicity in L929 cells evaluated by MTT assay.
RESULTSThe expression of sTNFRI in transfected cell culture supernatant was higher than control groups (P < 0.001). The expressed sTNFRI could significantly neutralize TNF-alpha cytotoxicity in L929 cells.
CONCLUSIONSThese results showed that the recombinant plasmid PcDNA3.1-sTNFRI can be expressed in mammalian cells and the recombinant sTNFRI has biological function.
