Effects of baicalin on the expression of pro-MMP-1 and MMP-3 in human gingival fibroblasts and periodontal ligament cells.
- Author:
Cheng-zhang LI
1
;
Zheng-guo CAO
;
Ru YANG
;
Zhu-huan SHANG
;
Li-jian JIN
;
E F COBERT
Author Information
- Publication Type:Journal Article
- MeSH: Collagenases; biosynthesis; genetics; Enzyme Precursors; biosynthesis; genetics; Fibroblasts; enzymology; pathology; Flavonoids; pharmacology; Gingiva; enzymology; pathology; Humans; Interleukin-1; pharmacology; Interleukin-1beta; Matrix Metalloproteinase 1; Metalloendopeptidases; biosynthesis; genetics; Peptide Fragments; pharmacology; Periodontal Ligament; enzymology; pathology; Periodontitis; enzymology; pathology; Scutellaria; chemistry
- From: Chinese Journal of Stomatology 2004;39(3):197-200
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the influence of baicalin on the IL-1beta induced pro-MMP-1 in HGF and the effects of baicalin on MMP-3 expression in periodontal ligament cells (PDLCs).
METHODSThe amount of secreted pro-MMP-1 and MMP-3 expression was detected by ELISA and cell immunochemistry.
RESULTS(1) The amount of secreted pro-MMP-1 (3.333 +/- 0.123) microg/L increased significantly following 1 microg/L of IL-1beta, compared with control group (1.960 +/- 0.180) microg/L. Addition of baicalin to cell culture medium for 1 hour following IL-1beta decreased pro-MMP-1 secretion in a dose-dependent manner in the range of 10 approximately 1,000 microg/L. (2) 1 microg/L IL-1beta could significantly stimulate the synthesis and secretion of MMP-3 in PDLCs. (3) The baicalin could not interfere the synthesis of MMP-3, but could inhibit the release of MMP-3 from PDLCs.
CONCLUSIONSBaicalin could inhibit the secretion of pro-MMP-1 and MMP-3 expression in IL-1beta induced HGF and PDLCs, which suggests that baicalin may play an important role in preventing and treating periodontal disease.
