Influence of S-nitrosoglutathione on agglutination and nitric oxide concentration in frozen platelets.
- Author:
Tao WU
1
;
Jing-Han LIU
;
Hui LI
;
Wu ZHOU
;
Shu-Ying WANG
Author Information
1. Department of Blood Transfusion, Chinese PLA General Hospital, Beijing, China.
- Publication Type:Journal Article
- MeSH:
Blood Platelets;
drug effects;
Freezing;
Humans;
Nitric Oxide;
metabolism;
Platelet Activation;
drug effects;
Platelet Aggregation;
drug effects;
Platelet Count;
S-Nitrosoglutathione;
pharmacology
- From:
Journal of Experimental Hematology
2012;20(2):386-389
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to investigate the influence of S-nitrosoglutathione (GSNO) on agglutination and nitric oxide (NO) concentration in frozen platelets. The agglutination of platelets was detected by using platelet agglutination apparatus, the level of NO in platelets was detected by the nitrate enzyme reduction method. The results showed that the rates of agglutination in freeze platelets and frozen platelets treated with GSNO were (35.47 ± 2.93) and (24.43 ± 3.07), which were significantly lower than that in fresh liquid platelets (63.44 ± 2.96). The level of NO concentration in frozen platelets was (22.16 ± 6.38), which was significantly lower than that in fresh liquid platelets (31.59 ± 16.88). The level of NO concentration in frozen platelets treated with GSNO was (45.64 ± 6.31), which was significantly higher than that in fresh liquid platelets (P < 0.01). It is concluded that GSNO increases the concentration of NO in frozen platelets, inhibits platelet activation and maintains platelet function, thus GSNO can be used as a frozen protective agent.
