Impact of clopidogrel on gene profile of human umbilical vein endothelial cell line and bioinformatics analysis.
- Author:
Xian-Feng LIU
1
;
Xue-Chun LU
;
Li FAN
;
Yan GAO
;
Cong MA
;
Yun LUO
Author Information
1. Department of Geriatric Hematology, General Hospital of Chinese PLA, Beijing, China.
- Publication Type:Journal Article
- MeSH:
Cell Line;
Human Umbilical Vein Endothelial Cells;
drug effects;
metabolism;
Humans;
Oligonucleotide Array Sequence Analysis;
RNA, Messenger;
genetics;
Ticlopidine;
analogs & derivatives;
pharmacology;
Transcription, Genetic;
Transcriptome
- From:
Journal of Experimental Hematology
2012;20(2):466-472
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to investigate the effect of clopidogrel on gene expression profile of cultured human umbilical vein endothelial cell (HUVEC) line (EA.hy926), and explore its potential molecule mechanism. A Affymetrix U133 plus 2.0 oligonucleotide microarray was applied to detect the alteration of gene expression profile induced by clopidogrel in HUVEC. Real time RT-PCR was used to verify the result of selected differentially expressing genes. The results showed that total 508 genes (including 139 up-regulated and 369 down-regulated genes) were obtained with differential expression more than 1.5-fold after clopidogrel (10 µmol/L) acted on HUVEC for 48 h. Clopidogrel affected the expression levels of genes involved protein binding, transcription factor activity, zinc ion binding, regulation of DNA-dependent transcription, transcription, RNA splicing and so on. It is concluded that the clopidogrel modulate function of endothelial cells by regulating sets of genes through different pathway.
