Expression of c-myc and c-myb gene in leukemic bone marrow stromal cells and their correlation.
- Author:
Ying ZHANG
1
;
Rui CHEN
;
Wei ZHANG
;
Juan LI
;
Xuan CHEN
;
Li ZHAO
Author Information
1. The First Hospital of Lanzhou University, Lanzhou, Gansu Province, China.
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Aged;
Aged, 80 and over;
Case-Control Studies;
Child;
Child, Preschool;
Female;
Gene Expression;
Gene Expression Regulation, Leukemic;
Genes, myb;
Genes, myc;
Humans;
Leukemia;
genetics;
metabolism;
Male;
Mesenchymal Stromal Cells;
metabolism;
Middle Aged;
Proto-Oncogene Proteins c-myb;
genetics;
metabolism;
Proto-Oncogene Proteins c-myc;
genetics;
metabolism;
Young Adult
- From:
Journal of Experimental Hematology
2012;20(3):531-535
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to investigate the expression level of oncogene c-myc and c-myb in leukemic cells and leukemic bone marrow stromal cells (BMSC) and the correlation with each other. The expression levels of c-myc and c-myb in those cells were examined semi-quantitatively by reverse transcription polymerase chain reaction (RT-PCR). Flow cytometry analysis was used to detect the membrane surface antigens of leukemic cells and BMSC. The karyotype was analyzed by G-banding techniques. The results showed that (1) c-myc and c-myb gene were expressed in the normal control group, the leukemic cells and BMSC of patients group. The mean expression levels of c-myb mRNA and c-myc mRNA in abnormal chromosomal leukemic cells were 1.03 ± 0.48 and 1.15 ± 0.38 respectively, which were significantly higher than those in control group (P < 0.05). In the abnormal karyotype stromal cells, the mean expression level of c-myb mRNA and c-myc mRNA were 2.08 ± 0.82 and 1.46 ± 0.29 respectively (P < 0.05). (2) The expression level of c-myc and c-myb mRNA were closely associated with patients' platelet counts (P < 0.05). (3) The expression of c-myc mRNA linearly correlated with the expression of c-myb mRNA in different prognostic groups. (4) In acute leukemic cells and BMSC, c-myc expression positively correlated with c-myb expression. (5) The expression level of c-myc in leukemic cells correlated with the expression levels of c-myc and c-myb in BMSC, respectively. It is concluded that the reduction of c-myc or c-myb expression levels may be a therapeutic regimen for leukemia.
