Gambogic acid induces apoptosis of Jurkat cell through the MAPK signal pathway.
- Author:
Yong XU
1
;
Jian OUYANG
;
Qi-Guo ZHANG
;
Min ZHOU
;
Juan LI
;
Min-Min CHEN
;
Yue-Yi XU
Author Information
1. Department of Hematology, Hospital of Nanjing University of Traditional Chinese Medicine, Jiangsu Province, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Caspase 3;
metabolism;
Caspase 9;
metabolism;
Humans;
JNK Mitogen-Activated Protein Kinases;
metabolism;
Jurkat Cells;
MAP Kinase Signaling System;
drug effects;
Xanthones;
pharmacology;
p38 Mitogen-Activated Protein Kinases;
metabolism
- From:
Journal of Experimental Hematology
2012;20(3):587-591
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to investigate the apoptosis-inducing effect of gambogic acid (GA) on Jurkat cells and its underlying signaling pathway. Apoptosis induced by GA and some inhibitors was assayed by Annexin V/PI doubling staining. The levels of caspase 3, caspase 8 and caspase 9 activated in living Jurkat cells were measured by flow cytometry. The expressions of caspase 3, caspase 9, p-JNK and P38 were detected by Western blot. The results showed that GA induced apoptosis of Jurkat cells in a dose-dependent manner. The positive cell number of activated caspase 3, caspase 8, caspase 9 and the levels of activated caspase 3, caspase 9, p-JNK, P38 increased after Jurkat cells were treated with GA. ROS, CaMKII, caspase 3, caspase 9, MAPKK, JNK1/2 and P38 inhibitors had some significant effect on GA-induced apoptosis. ROS, CaMKII, MAPKK, JNK1/2 and P38 inhibitors decreased the levels of activated caspase 3, caspase 9 by GA.ROS, CaMKII, MAPKK, JNK1/2 inhibitors decreased the levels of p-JNK by GA. ROS, CaMKII, MAPKK, P38 inhibitors decreased the levels of P38 by GA. It is concluded that GA induced apoptosis of Jurkat cells by activated caspases through activating of ROS-CaMKII-MAPKK-JNK/P38 pathway.
