Longikaurin A induces apoptosis of multiple myeloma H929 cells.
- Author:
Shan ZHAO
1
;
Jian-Xin PU
;
Han-Dong SUN
;
Ying-Li WU
Author Information
1. Department of Pathophysiology, Shanghai Jiaotong University School of Medicine, Shanghai, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Caspase 3;
metabolism;
Caspase 9;
metabolism;
Cell Line, Tumor;
Diterpenes, Kaurane;
pharmacology;
Humans;
Multiple Myeloma;
pathology;
Reactive Oxygen Species;
metabolism
- From:
Journal of Experimental Hematology
2012;20(3):611-615
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to investigate the biological effect of longikaurin A on multiple myeloma H929 cells. Effects of oridonin and longikaurin A on proliferation of H929 cells were evaluated by CCK-8 assay. Cell morphological features of H929 cells were examined under inverted phase contrast microscope. Apoptosis was determined by Annexin V/PI staining. Expression of caspase-3, caspase-9, and PARP were detected by Western blot. Reactive oxygen species (ROS) level was determined by DCFDA assay. The results showed that longikaurin A (IC(50) 0.85 µmol/L) was more effective than oridonin ((IC(50) 10.66 µmol/L) to inhibit the proliferation of H929 cells. Treatment with longikaurin 2 µmol/L significantly increased the percentage of Annexin V positive cells. Caspase-3 and caspase-9 were activated and PARP, one substrate of caspase-3, was cleaved into 85 kDa fragments. The ROS scavenger N-acetyl-cysteine significantly blocked apoptosis induced by longikaurin A. However, longikaurin A did not increase the ROS level in H929 cells. It is concluded that longikaurin A is more effective than oridonin to induce apoptosis in multiple myeloma H929 cells without increasing the ROS level.
