Genotype analysis of RhD-negative donors with immune antibodies.
- Author:
Chun-Yan ZHANG
1
;
Ji-Hong LI
;
Su-Zen ZHAO
;
Jie LIU
Author Information
1. Harbin City Blood Genter, Harbin, Heilongjiang Province, China.
- Publication Type:Journal Article
- MeSH:
Base Sequence;
Blood Donors;
Exons;
Genotype;
Humans;
Isoantibodies;
Phenotype;
Rh-Hr Blood-Group System;
genetics;
immunology;
Rho(D) Immune Globulin
- From:
Journal of Experimental Hematology
2012;20(3):753-756
- CountryChina
- Language:Chinese
-
Abstract:
In order to analyze the genotype of RhD-negative blood donors with immune antibodies in Harbin, the voluntary blood donors from 1 April 2008 to 30 september 2011 were detected serologically to determine the RhD-negative donors. The blood donors confirmed to be RhD negative were detected to screen the immune antibodies, the samples with immune antibodies were analyzed by PCR-SSP and DNA sequencing to detect RhD genotype. The results showed that the 12 cases of the immune antibodies (0.95%) were screened out from 1265 cases of RhD-negative donors, among which 9 cases showed anti-D-antibody, 3 cases showed anti-(D+C) antibody; 10 cases were RhD-negative, 2 cases were RHD 711D(el)C. It is concluded that RhD negative and RHD 711D(el)C are easy to be immunized to produce the immune antibodies; RhD-negative population, especially women should be highly aware of avoiding mis-transfusion of RhD-positive blood, and also avoiding multiple pregnancies resulting in newborn's hemolytic disease.
