Immunomodulatory function of orally administered thymosin alpha1.
- Author:
Xiang-Ming CHEN
1
;
Han-Liang JIANG
;
Lin-Fu ZHOU
;
Xiao-Ping PAN
;
Zhong-Rong HU
;
Rong-Hua LIU
;
Xiao-Ming CHEN
;
Zhi CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Administration, Oral; Animals; CD4-Positive T-Lymphocytes; drug effects; CD8-Positive T-Lymphocytes; drug effects; Cyclophosphamide; toxicity; Cytokines; metabolism; Immunologic Deficiency Syndromes; chemically induced; drug therapy; immunology; Immunologic Factors; administration & dosage; genetics; Immunosuppressive Agents; toxicity; Mice; Mice, Inbred BALB C; Recombinant Proteins; administration & dosage; genetics; Saccharomyces cerevisiae; genetics; Thymosin; administration & dosage; analogs & derivatives
- From: Journal of Zhejiang University. Science. B 2005;6(9):873-876
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the immunological function of a yeast expression system for thymosin alpha1 (Talpha1).
METHODSA constructed Talpha1 yeast expression system was used to investigate the immunological function of orally administered Talpha1. Dried yeast containing three different concentration of Talpha1 was fed to normal Balb/c mice and other Balb/c mice whose immunities were inhibited in advance by cyclophosphamide. Synthesized Talpha1 peptide was used as positive control and dried yeast with empty plasmid was used as negative control. CD4(+) and CD8(+) levels were detected by flow cytometry assay. TNF-alpha, IFN-gamma, IL-2, IL-6 and IL-10 levels were detected by liquid chip.
RESULTSIn normal Balb/c mice or immune inhibition Balb/c mice, CD8(+) levels were significantly increased. Especially in immune inhibition Balb/c mice, CD8(+) levels in synthesized Talpha1 group (18.77%+/-4.72%), small dose group (13.48%+/-6.17%) and large dose group (22.74%+/-1.09%) were significantly higher than that in empty yeast control group (7.49%+/-2.14%).
CONCLUSIONOrally administered Talpha1 has its certain immunomodulatory function.
