In vitro study of immunosuppressive effect of apoptotic cells.
- Author:
Wen-Jin ZHANG
1
;
Shu-Sen ZHENG
Author Information
1. Department of Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310027, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Antigens, CD;
metabolism;
Antigens, Differentiation, T-Lymphocyte;
metabolism;
Apoptosis;
immunology;
Chemokine CXCL2;
Chemokines;
biosynthesis;
genetics;
Concanavalin A;
pharmacology;
Cytokines;
biosynthesis;
Female;
Humans;
Immune Tolerance;
In Vitro Techniques;
Jurkat Cells;
Lectins, C-Type;
Lipopolysaccharides;
pharmacology;
Lymphocyte Activation;
drug effects;
Macrophages;
drug effects;
immunology;
Mice;
Mice, Inbred ICR;
Phagocytosis;
Receptors, Interleukin-2;
metabolism;
Signal Transduction;
immunology;
T-Lymphocytes;
drug effects;
immunology;
Tumor Necrosis Factor-alpha;
biosynthesis;
genetics
- From:
Journal of Zhejiang University. Science. B
2005;6(9):919-925
- CountryChina
- Language:English
-
Abstract:
Recent studies revealed that apoptotic cells are actively involved in immunosuppression and anti-inflammation. After being phagocytosed by macrophages, apoptotic cells can actively regulate cytokines secretion from lipopolysaccharide (LPS)-stimulated macrophages, in which the secretion of immunosuppressive cytokines such as interleukin-10 (IL-10) is increased while the pro-inflammatory cytokines such as tumor necrosis factor-alpha (TNFa), interleukin-1beta (IL-1b) and leukin-8 (IL-8) are suppressed. In this paper, we first present evidence that phagocytosed apoptotic cells regulate cytokine secretion of LPS-stimulated macrophages, but also inhibit the activation of T lymphocytes stimulated by ConA. These data suggest that apoptotic cells can alter the biological behavior of macrophages which gain immunosuppressive property.
