Investigation on the origin of 5-HMF in Shengmaiyin decoction by RP-HPLC method.
- Author:
Ying-Hua LI
1
;
Xiu-Yang LU
Author Information
1. Institute of Pharmaceutical Engineering, Zhejiang University, Hangzhou 310027, China.
- Publication Type:Journal Article
- MeSH:
Chromatography, High Pressure Liquid;
Drug Combinations;
Drugs, Chinese Herbal;
chemistry;
Fructose;
metabolism;
Furaldehyde;
analogs & derivatives;
chemistry;
isolation & purification;
Hydrogen-Ion Concentration;
Schisandra;
chemistry
- From:
Journal of Zhejiang University. Science. B
2005;6(10):1015-1021
- CountryChina
- Language:English
-
Abstract:
The origin of 5-HMF (5-hydroxymethyl-2-furaldehyde) in a Shengmaiyin decoction was investigated by the RP-HPLC method below. A C(18) column (250 mm x 4.6 mm, i.d. 5 microm) with a column temperature of 25 degrees C was used. The mobile phase was a mixture of ultra-pure water-acetonitrile (95:5, V/V) and the flow rate was 1.0 ml/min. The detection wavelength was 280 nm. The injection volume was 1 microl and the running time was about 20 min. The addition of Schisandra was regulated to assess the contribution of an acid environment to the production of 5-HMF. In order to confirm the role of saccharides in the production of 5-HMF, different amount of fructose was used. The 5-HMF level in decoctions of processed and unprocessed Schisandra was investigated in order to determine the origin of 5-HMF. The results showed that 5-HMF was derived mainly from the decoction of Schisandra only and not the mixed decoction of Ophionpogon and Schisandra. The appearance of 5-HMF is not simply the result of the decomposition of saccharides under the acid environment created by Schisandra, but the processing procedure plays an important role in the production of 5-HMF.
