Determination of theophylline concentration in serum by chemiluminescent immunoassay.
- Author:
Mei-xia ZHOU
1
;
Cha-ying GUAN
;
Guang CHEN
;
Xin-you XIE
;
Sheng-hai WU
Author Information
- Publication Type:Journal Article
- MeSH: Blood Chemical Analysis; methods; Female; Fluorescence Polarization Immunoassay; methods; Humans; Luminescent Measurements; methods; Lung Diseases; blood; Male; Middle Aged; Reproducibility of Results; Sensitivity and Specificity; Theophylline; blood
- From: Journal of Zhejiang University. Science. B 2005;6(12):1148-1152
- CountryChina
- Language:English
-
Abstract:
OBJECTIVEThis study aimed to establish chemiluminescent immunoassay (CLIA) for quantitative determination of theophylline levels in human serum.
METHODSTo measure the concentration of theophylline (n=122) and evaluate the assay.
RESULTSThe linear range of the CLIA method was 0.51-40 mg/L (Y=1.02X+0.44, r=0.995). The intra and inter CV (coefficient variance) of CLIA were 3.20% and 3.57%, respectively. The average recovery rate was 102.3%. This method was free from interference by brilirubin (<200 micromol/L), hemoglobin (<10 g/L), and triglycerides (<15 mmol/L).
CONCLUSIONThis method is simple, convenient and precise for clinical pharmacokinetics study of theophylline.
