Promoter trapping in Magnaporthe grisea.
- Author:
Xiao-Hong LIU
1
;
Jian-Ping LU
;
Jiao-Yu WANG
;
Hang MIN
;
Fu-Cheng LIN
Author Information
1. Biotechnology Institute, School of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China. minhang@zju.edu.cn
- Publication Type:Journal Article
- MeSH:
Fungal Proteins;
biosynthesis;
genetics;
Gene Expression Regulation, Fungal;
genetics;
Genes, Reporter;
genetics;
Genetic Vectors;
genetics;
Green Fluorescent Proteins;
genetics;
metabolism;
Magnaporthe;
genetics;
metabolism;
Promoter Regions, Genetic;
genetics;
Protein Engineering;
methods;
Recombinant Proteins;
metabolism
- From:
Journal of Zhejiang University. Science. B
2006;7(1):28-33
- CountryChina
- Language:English
-
Abstract:
Application of promoter trapping based on transformation in Magnaporthe grisea is reported in this paper. Two promoter-trapping vectors, designated as pCBGFP and pEGFPHPH, were constructed and transformed into protoplasts of M. grisea. A library of 1,077 transformants resistant to hygromycin B was generated. Of which, 448 transformants were found to express eGFP gene in different structures of M. grisea. Three transformants grew slowly, 5 transformants decreased in conidiation and 7 transformants reduced in pathogenicity greatly among these 448 transformants. Eleven transformants were checked by genomic southern blot randomly, and 9 of which were single-copy insertions. The promoter trapping technique has been applied successfully in M. grisea and can be used as a tool for functional genomic analysis.
