RAPD analysis for genetic diversity of Fagopyrum cymosum.

Author: Chunping ZHANG ¹ ; Ping HE ; Shijun HU ; Yifeng ZHANG ; Huacun JI ; Shan GAO
Author Information

1. School of Life Sciences, Southwest University, Key Laboratory (Ministry of Education) of Eco-environments of Three Gorges Reservoir Region, Chongqing Key Laboratory of Plant Ecology , Chongqing, China.
Publication Type:Journal Article
MeSH: Cluster Analysis; Fagopyrum; genetics; Genetic Variation; Phylogeny; Random Amplified Polymorphic DNA Technique
From: China Journal of Chinese Materia Medica 2009;34(6):660-663
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo study the genetic diversity of Fagopyrum cymosum.
METHODThe genetic diversity of 87 individuals from 10 populations was analyzed by random amplified polymorphic DNA (RAPD).
RESULTTwelve primers were selected to produce highly reproducible RAPD bands. Among 85 amplified bands, seventy-five showed polymorphism, the percentage of polymorphic bands reached to 88.24%. Nei's gene diversity index (H) was 0. 3103, Shannon information index (I) was 0.5632, Gst was 0.1924. The genetic similarity coefficient and the genetic distance were 0.6720-0.9678 and 0.0328-0.3975, respectively.
CONCLUSIONF. cymosum shows high genetic diversity and the majority of genetic variation occurs in populations. By cluster analysis, the geographical distribution is very obvious. The RAPD marker can be used for the analysis of the genetic diversity and genetic variation of F. cymosum.