Suppressive effect of resveratrol on growth of U251 human glioma cells and its correlated mechanism.
- Author:
Hongsheng LIU
1
;
Jinhuan WANG
;
Xinnv XU
;
Na LIN
;
Biao ZHANG
;
Xuequan FENG
;
Zhongqiu LUO
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; Blotting, Western; Caspase 3; metabolism; Cell Line, Tumor; Cyclin D1; metabolism; Gene Expression Regulation, Neoplastic; drug effects; Glioma; drug therapy; metabolism; Humans; Immunohistochemistry; Proto-Oncogene Proteins c-bcl-2; metabolism; STAT3 Transcription Factor; metabolism; Stilbenes; therapeutic use; bcl-2-Associated X Protein; metabolism; bcl-X Protein; metabolism
- From: China Journal of Chinese Materia Medica 2009;34(8):1027-1031
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the suppressive effect of resveratrol on growth of U251 human glioma cells and its correlated mechanism.
METHODU251 human glioma cells were treated with resveratrol at various concentrations, MTT assay was used to determine the inhibitory rate of cell proliferation, FCM to detect the cell apoptosis, the expressions of Bcl-2, Bcl-XL, STAT3 and CyclinD1 were analysed by immunohistochemistry and Western blot to examine the expression of Bcl-2, Bcl-XL, STAT3, CyclinD1, Caspase-3 and Bax.
RESULTAfter treatment with resveratrol, MTT assay showed the growth of U251 cells was inhibited in dose-dependent and time-dependent manners, apoptosis of cells advanced stage was built up, immunohistochemical staining displayed decreased the expression of Bcl-2, Bcl-XL, STAT3 and CyclinD1 and Western blot showed that resveratrol decreased the expression of Bcl-2, Bcl-XL, STAT3 and CyclinD1, and built up Bax and Caspase-3.
CONCLUSIONIt is possible that downregulated the expression of Bcl-2, Bcl-XL, but upregulated Bax and Caspase-3, and the indication was obviously in dose-dependent and time-dependent manners.
