Effect of Panax notoginseng saponins on syp and tau gene expression in brain of senescence accelerated mouse.
- Author:
Liang LV
1
;
Zhenguo ZHONG
;
Dengpan WU
;
Limin CHAI
;
Wenyan ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Aging; drug effects; metabolism; Animals; Brain; drug effects; metabolism; Gene Expression; drug effects; genetics; Mice; Panax notoginseng; chemistry; Protein Tyrosine Phosphatase, Non-Receptor Type 11; genetics; metabolism; Saponins; pharmacology; tau Proteins; genetics; metabolism
- From: China Journal of Chinese Materia Medica 2009;34(10):1261-1263
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of Panax notoginseng saponins (PNS) on (synaptophysin, syp) and tau gene expression in the brain tissue in senescence accelerated mouse prone 8 (SAMP 8).
METHODSAMP8 were randomly divided into 4 groups: PNS 23.38, 93.50 mg x kg(-1) group, huperzin A 0.038 6 mg x kg(-1) x d(-1) group and blank control group; the drug groups were treated with the designed drugs respectively per day by intragastric administration for 4 consecutive weeks, and double distilled water was given to blank control group. After treatment, the mRNA content of tau and syp were assayed by reverse transcription (RT) and real-time polymerase chain reaction (real-time PCR).
RESULTCompared with blank control group, the syp mRNA contents were increased in PNS groups (P < 0.05 or P < 0.01), and the tau mRNA content were not significant difference in all groups.
CONCLUSIONThis study suggests that PNS can up-regulate syp gene expression at transcriptional level in the brain of SAMP 8.
