Research on callus induction and cell suspension culture of Changium smyrnioides.
- Author:
Shu JIANG
1
;
Jin'ao DUAN
;
Jianwei CHEN
;
Jinhua TAO
;
Kunhua FU
Author Information
- Publication Type:Journal Article
- MeSH: Cell Culture Techniques; methods; Dose-Response Relationship, Drug; Magnoliopsida; cytology; drug effects; physiology; Plant Growth Regulators; pharmacology; Plant Leaves; cytology; drug effects; physiology; Time Factors
- From: China Journal of Chinese Materia Medica 2009;34(9):1078-1081
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish the technical systems of callus induction and the cell suspension culture of Changium smyrnioides.
METHODEffects of different plant growth regulators on leaf, petiole and root callus induction were studied by orthogonal design, and cell suspension cultures from different explants were carried out.
RESULTPetiole was optimal to form callus, followed by the root and leaf. Four kinds of plant growth regulators showed a significant effect on the petiole and root callus induction. The optimal plant growth regulators for petiole callus induction was 0.5 mg x L(-1) NAA, 0.5 mg x L(-1) 2,4-D, 0.1 mg x L(-1) KT and 0.5 mg x L(-1) 6-BA; and induction rate reached to 100% when petiole was cultured for 14 days. In addition, when cell from petiole callus was cultured for 21 days, the maximum yields of cell biomass and polysaccharide were 1090.28 mg x L(-1) and 36.85 mg x L(-1), respectively.
CONCLUSIONPetiole was taken as the optimal explant for inducing callus and the optimal cell origin for the cell suspension culture of C. smyrnioides.
