Determination of content and entrapment efficiency of 20 (S)-protopanaxadiol in pharmacosomes by RP-HPLC method.
- Author:
Meihua HAN
1
;
Jing CHEN
;
Shilin CHEN
;
Xiangtao WANG
Author Information
- Publication Type:Journal Article
- MeSH: Calibration; Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; chemistry; Panax; chemistry; Reproducibility of Results; Sapogenins; analysis; isolation & purification
- From: China Journal of Chinese Materia Medica 2009;34(9):1082-1085
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a RP-HPLC method for content and entrapment efficiency of 20 (S)-protopanaxadiol in pharmacosomes.
METHODThe separation was performed with a COSMOSIL 5 C18-MS-II column (4.6 mm x 250 mm, 5 mmicrom) using methanol-water (95:5) as the mobile phase and detected at 203 nm. The flow rate was 1.0 mL x min(-1) and 50 microL sample solution was injected for each time.
RESULTThe calibration curve was linear within the range 0.1-0.5 mg x mL(-1) (r = 0. 9999) , the intra-day RSD and inter-day RSD were less than 2% and the average recovery was between 101.44%-103.11% (n = 3).
CONCLUSIONThe method is simple, accurate, sensitive and applicable for determination of content and entrapment efficiency of 20 (S)-protopanaxadiol pharmacosomes.
