Alpha-glucosidase inhibitors from Rubia cordifolia.
- Author:
Wenyi KANG
1
;
Li ZHANG
;
Yanli SONG
Author Information
- Publication Type:Journal Article
- MeSH: Binding, Competitive; Enzyme Inhibitors; analysis; metabolism; pharmacology; Glycoside Hydrolase Inhibitors; Inhibitory Concentration 50; Kinetics; Rubia; chemistry; alpha-Glucosidases; metabolism
- From: China Journal of Chinese Materia Medica 2009;34(9):1104-1107
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo search alpha-glucosidase inhibitors from Rubia cordifolia.
METHODThe alpha-glucosidase inhibitors were isolated by the column chromatographic techniques and the bioassay-guided method in vitro. A combination of MS and NMR spectroscopy was used to identify the chemical structures. The inhibitory kinetics of the inhibitors were also investigated.
RESULTThe chloroform extract showed high inhibitory activity, and three active compounds were isolated and identified as 1,3-dihydroxy-2-methylanthraquinone (1), 1-hydroxy-2-methylanthraquinone (2) and 1,2-dihydroxyanthraquinone (3). The IC5o values of compound 1-3 were all lower than that of acarbose. Compound 1 and 2 shown competitive type manner on alpha-glucosidase, whereas compound 3 shown noncompetitive type model.
CONCLUSIONCompounds 1-3 as strong inhibitors of alpha-glucosidase were reported for the first time.
