Tyrosinase inhibitor from Angelica dahurica roots.
- Author:
Xianglan PIAO
1
Author Information
- Publication Type:Journal Article
- MeSH: Angelica; chemistry; Chromatography, High Pressure Liquid; Enzyme Inhibitors; analysis; isolation & purification; pharmacology; Inhibitory Concentration 50; Monophenol Monooxygenase; antagonists & inhibitors; Plant Roots; chemistry
- From: China Journal of Chinese Materia Medica 2009;34(9):1117-1120
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo isolate and identify the tyrosinase inhibitory constituent from Angelica dahurica.
METHODA tyrosinase inhibitory constituent was isolated by silica gel column chromatography combined with a bioassay-linked HPLC method.
RESULTAn extract of heat-processed A. dahurica roots showed more potent tyrosinase inhibitory activity than that of the crude drug. Tyrosinase inhibitory furanocoumarin, 8-hydroxy-5-methoxypsoralen,was isolated. It express stronger tyrosinase inhibition than the known tyrosinase inhibitor, kojic acid (IC50 = 0.0907 mmol x L(-1)) with IC50 value of 0.0086 mmol x L(-1). Therefore, it is contributive to the increased tyrosinase inhibitory activity of the heat-processed A. dahurica.
CONCLUSIONThe active constituent was 8-hydroxy-5-methoxypsoralen from the heat-processed A. dahurica.
