Simultaneous determination of three coumarin constituents in roots of Peucedanum praeruptorum by RP-HPLC.
- Author:
Wenling WU
1
;
Jiajia CHEN
;
Shoujin LIU
;
Nianjun YU
;
Chengwu FANG
;
Qingqing ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Apiaceae; chemistry; Chromatography, High Pressure Liquid; Coumarins; analysis; isolation & purification; Linear Models; Plant Roots; chemistry; Reproducibility of Results; Time Factors
- From: China Journal of Chinese Materia Medica 2009;34(9):1121-1123
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a reserved-phase HPLC method for the determination of praeruptorin A, praeruptorin B, qianhucoumarin E in roots of Peucedanum praeruptorum.
METHODAgilent TC-C18 column (4.6 mm x 250 mm, 5 microm) was used at 30 degrees C with the mobile phase of methanol-water (75:25). The flow rate was set at 0.8 mL x min(-1). The detection wavelength was 321 nm.
RESULTThe linear response ranged from 3.20-28.80 microg for +/- praeruptorin A (r = 0.9999, n = 5), 1.60-14.40 g for praeruptorin B (r = 0.9995, n = 5) and 1.64-14.76 g for qianhucoumarin E (r = 0.9994, n = 5), respectively. Recoveries were 98.92% with RSD 1.6% for praeruptorin A, 99.66% with RSD 1.5% for praeruptorin B and 99.72% with RSD 1.4% for qianhucoumarin E.
CONCLUSIONThe method is quick, simple and repeatable for determination of three coumarin constituents in root of P. praeruptorum.
