Expression of tissue transglutaminase on renal interstitial fibrosis rats and intervention of GBE.
- Author:
Dan WANG
1
;
Xiangjun LI
;
Xiaoyan YU
;
Yan SHI
;
Li YIN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Drugs, Chinese Herbal; pharmacology; Fibrosis; Gene Expression Regulation, Enzymologic; drug effects; Ginkgo biloba; chemistry; Immunohistochemistry; Kidney; drug effects; enzymology; metabolism; pathology; Male; RNA, Messenger; genetics; metabolism; Rats; Rats, Wistar; Transglutaminases; genetics; metabolism
- From: China Journal of Chinese Materia Medica 2009;34(9):1133-1136
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of tissue transglutaminase( tTG) in unilateral ureteral obstruction (UUO) rats and the intervention of Folium Ginkgo (GBE).
METHODThe animal models of unilateral ureteral obstruction (UUO) were used. Wistar rats were randomly divided into four groups, the Sham-operated group, the UUO group, the benazepril (Bena)-treated UUO group and the GBE-treated group. The rats were sacrificed at day 14. Histological changes in renal tubular interstitium were observed with HE and Masson staining, and the mRNA and protein levels of tTG and FN were detected by RT-PCR and immunohistochemistry.
RESULTCompared with the sham group, the expression of tTG and FN were significantly increased (P < 0.01), and decreased after been treated by Bena and GBE (P < 0.05).
CONCLUSIONBena and GBE may suppress the development of fibrosis partially via down-regulation of tTG expression.
