Study on fast quality control in extracting process of Paeonia lactiflora using near infrared spectroscopy.
- Author:
Xueying CHEN
1
;
Yerui LI
;
Yong CHEN
;
Longhu WANG
;
Changhai SUN
;
Xuesong LIU
Author Information
- Publication Type:Journal Article
- MeSH: Paeonia; chemistry; Plant Extracts; chemistry; Quality Control; Spectroscopy, Near-Infrared; methods
- From: China Journal of Chinese Materia Medica 2009;34(11):1355-1358
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo set up a novel method for fast analysis of active components in water extracting process of Paeonia lactiflora with near infrared spectroscopy (NIRS).
METHODHPLC was used as the reference method to determine the content of Paeoniflorin and a multivariate calibration model based on PLS algorithm was developed to analyze the correlation between the spectra and the corresponding values determined by the reference method.
RESULTThe correlation coefficient of the calibration model was 0.996 2, and the predicted coefficient was 0.989 5. The RMSEC and RMSEP were 0.109 g x L(-1) and 0.138 g x L(-1), respectively, and the RSEP was 5.6%.
CONCLUSIONThe method mentioned above is proved to be convenient, rapid and nondestructive, accurate and reliable, and is applicable for fast analysis and monitoring of active components in extraction process of traditional Chinese medicine.
