Expression of GATA-1 and GATA-2 in the bone marrow of patients with Monge's disease.
- Author:
Jian-ping LI
1
;
Nai-yong JIA
;
Zhan-quan LI
;
Hong-xin WANG
;
Juan SU
;
Yu-li LI
;
Xue-mei LI
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Altitude Sickness; metabolism; GATA1 Transcription Factor; metabolism; GATA2 Transcription Factor; metabolism; Humans; Male; Polycythemia; metabolism; RNA, Messenger; metabolism
- From: Chinese Journal of Hematology 2007;28(8):537-540
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo find out how GATA-1 and GATA-2 behave in the bone marrow of patients with Monge's disease.
METHODSThe levels of mRNA in mononuclear cells (MNC) and proteins of GATA-1 and GATA-2 in the bone marrow of patients with Monge's disease and controls were determined by RT-PCR and immune cytolysis chemical method.
RESULTS(1) All patients and controls expressed GATA-1 mRNA (Monge's disease 1.033 +/- 0.146, Control 0.458 +/- 0.076) and GATA-2 mRNA (Monge's disease 0.451 +/- 0.073, Control 0.185 +/- 0.074). All patients expressed both GATA-1 (positive cell counts 77.3 +/- 33.3, positive score 135.4 +/- 75.4) and GATA-2 ( positive cell counts 29.4 +/- 11.4, positive score 48.4 +/- 19.7). All the controls expressed GATA-1 (positive cell counts 18.1 +/- 11.3, positive score 24.2 +/- 13.4) while 12 of 20 controls expressed GATA-2 ( positive cell counts 5.4 +/- 3.0, positive score 7.3 +/- 4.2). The expression of mRNA and proteins of GATA-1 and GATA-2 in Monge's disease were higher than in controls (P < 0.01). (2) There was a positive correlation between GATA-1 and Hb (P < 0.01), as did between mRNA and proteins of GATA-1 and GATA-2. (3) Both the proteins of GATA-1 and GATA-2 located only in the cytoplasm but not the nucleus.
CONCLUSIONSTwo of inherent genes, GATA-1 and GATA-2 which were expressed at higher levels in patients with Monge's disease than in controls might play significant roles in the pathogenesis of Monge's disease.
