Application of flow cytometric immunophenotypic analysis in the diagnosis of malignant lymphoma.
- Author:
Li-Qun REN
1
;
Li-Yan XUE
;
Rui BI
;
Jian-Ming LIANG
;
Dong-Mei LIN
;
Jie MA
;
Ning LÜ
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; Child; Female; Flow Cytometry; Humans; Immunophenotyping; methods; Lymphoma; diagnosis; immunology; pathology; Male; Middle Aged
- From: Chinese Journal of Hematology 2007;28(10):671-676
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the value of flow cytometric immunophenotyping (FCI) in the diagnosis and differentiated diagnosis of lymphoma and explain the immunophenotypic features and differences of malignant lymphoma.
METHODSSeventy four fresh samples of suspicious lymphoma were collected from Nov. 2004 to Aug. 2006. Each sample was individually evaluated by FCI. The results were analyzed and compared with the histopathological diagnosis.
RESULTSAmong the 74 cases, the FCI data consisted with the final morphological diagnosis in 61 cases (82.4%). For the diagnosis of B and T non-Hodgkin's lymphoma (NHL), thymoma, carcinoma and benign lesions of lymph node, the concordance between FCI data and morphological diagnosis were 93.5%, 100%, 100%, 100% and 81.3%, respectively.
CONCLUSIONMulti-parameter FCI analysis can provide important information and help for diagnosis of lymphoma. It is an assistant but necessary approach for the diagnosis and differential diagnosis of lymphoma.
