Expression and mutation of myc antagonist genes Mad1, Mxi1 and Rox in leukemia cells.
- Author:
Xiao-Hui SUO
1
;
Ling PAN
;
Li YAO
;
Xue-Jun ZHANG
;
Zhi-Yun NIU
;
Zuo-Ren DONG
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; genetics; metabolism; Basic Helix-Loop-Helix Transcription Factors; genetics; metabolism; Cell Cycle Proteins; genetics; metabolism; Female; Humans; Leukemia; genetics; metabolism; Male; Middle Aged; Mutation; Nuclear Proteins; genetics; metabolism; Polymorphism, Single-Stranded Conformational; Repressor Proteins; genetics; metabolism; Tumor Suppressor Proteins; genetics; metabolism
- From: Chinese Journal of Hematology 2007;28(11):745-749
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression and mutation of Mad1, Mxi1 and Rox genes in leukemia cells.
METHODSExpression and mutation of Mad1, Mxi1 and Rox genes in bone marrow mononuclear cells (BMMNC) from 26 de novo acute leukemia (AL) patients, and in peripheral blood mononuclear cells (PBMNC) from 30 healthy volunteers, as well as in 7 human leukemic cell lines were analyzed by reverse transcription-polymerase chain reaction (RT-PCR), single strand conformational polymorphism (SSCP) and DNA sequencing.
RESULTSRT-PCR showed that all the above cells expressed Mad1, Mxi1 and Rox mRNA. SSCP revealed four polymorphisms: two in Mad1, one each in Mxi1 and Rox. DNA sequencing detected nine missense mutations: two in Mad1 in AL patients, four in Mxi1 (three in AL patients and one in KG-1 cell line), and three in Rox in AL patients. The mutations of Mad1, Mxi1 and Rox mRNA were detected in 2, 3 and 3 patients, respectively.
CONCLUSIONIt is for the first time to demonstrate the mutations of Mad1, Mxi1 and Rox genes in AL patients suggesting these mutated genes involve in the pathogenesis of leukemia.
