Utilization of the stable ectopic expression cell line as a model for the investigation of RIG-G gene.
- Author:
Shu XIAO
1
;
Pei-min JIA
;
Man-gen SONG
;
Dong LI
;
Xiao-rong PAN
;
Zhu CHEN
;
Jian-hua TONG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Cycle; genetics; Cell Differentiation; Cell Proliferation; Cyclin-Dependent Kinase Inhibitor p21; genetics; metabolism; Cyclin-Dependent Kinase Inhibitor p27; genetics; metabolism; Humans; Intracellular Signaling Peptides and Proteins; genetics; physiology; Plasmids; genetics; Transfection; U937 Cells
- From: Chinese Journal of Hematology 2007;28(12):795-798
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the biological function of RIG-G gene by establishing a cell line stably expressing RIG-G protein.
METHODSEctopic RIG-G gene was transfected into U937 cells by using Tet-off expression system. Changes before and after RIG-G expression were detected for cell growth, cell morphology, cell surface antigen and cell cycle regulating proteins.
RESULTSRIG-G protein arrested the cells at G0/G1 phase and inhibited cell growth by increasing the cell cycle inhibitors P21 and P27. As compared to that in control group, the proportion of cells at G0/G1 phase in RIG-G-expressing cell group increased from (43.9 +/- 5.6)% to (63.9 +/- 2.3)% (P < 0.01). The rate of growth inhibition was (68.7 +/- 0.2)%. In addition, an increase in CD11C expression [(61.3 +/- 1.1)% vs. (18.0 +/- 0.4)% (P < 0.01)] and in cells with morphologic features of partial differentiation (smaller cell size, reduced nucleus-cytoplasm ratio, notched nucleus and coarse chromatin) was also observed in RIG-G-expressing cells.
CONCLUSIONSRIG-G has potential abilities to inhibit cell proliferation and promote cell differentiation.
