N-ras and fms gene mutation in idiopathic thrombocytopenic purpura and myelodysplasia.
- Author:
Hong-Yu ZHAO
1
;
Ming HOU
;
Xiao-Fang LI
;
Dao-Xin MA
;
Qi-Ji LIU
;
Pin WANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Female; Genes, fms; genetics; Genes, ras; genetics; Humans; Male; Middle Aged; Myelodysplastic Syndromes; genetics; Point Mutation; Polymerase Chain Reaction; Polymorphism, Single-Stranded Conformational; Purpura, Thrombocytopenic, Idiopathic; genetics
- From: Chinese Journal of Hematology 2008;29(3):158-160
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the pathogenesis of idiopathic thrombocytopenic purpura (ITP) and improve the differential diagnosis from myelodysplastic syndromes (MDS).
METHODSPolymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) was performed to detect the point mutation of codon 12,13 in N-ras gene and codon 301, 969 in fms gene in adult and aged ITP and MDS patients.
RESULTSIn 25 ITP patients, N-ras mutation and fms mutation were detected in one each (4%). Mutations were found in 3 of 8 MDS patients: two (25%) with N-ras mutation and one (12.5%) with fms mutation.
CONCLUSIONSPatients with N-ras or fms gene mutation diagnosed as MDS rather than ITP.
