IgVH mutation status in patients with chronic lymphocytic leukemia.
- Author:
Ya-Ping ZHANG
1
;
Li-Juan CHEN
;
Wen-Juan ZHENG
;
Yu-Jie WU
;
Hong-Xia QIU
;
Si-Xuan QIAN
;
Wei XU
;
Jian-Yong LI
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Aged, 80 and over; Chronic Disease; Female; Humans; Immunoglobulin Heavy Chains; genetics; Immunoglobulin Variable Region; genetics; Leukemia, Lymphocytic, Chronic, B-Cell; genetics; Male; Middle Aged; Mutation
- From: Chinese Journal of Hematology 2008;29(3):164-167
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the frequency and mutation status of IgVH gene expression in patients with chronic lymphocytic leukemia (CLL) in China.
METHODSIgVH mutation was detected by multiplex PCR and directly sequencing in 29 CLL patients. IgH somatic hypermutation and mutation site were analysed by IMGT/V-QUEST.
RESULTSOf 29 CLL patients, 21 had IgVH mutation (72%). The most frequently expressed VH gene family was found to be VH3 (55%) followed by VH4 (38%), VH2 (3.5%) and VH7 (3.5%), with no expression of VH1, VH5 and VH6.
CONCLUSIONSThe expression frequency of IgVH gene families in Chinese CLL patients is significantly different from that in Western CLL patients, suggesting the involvement of ethnic and/or environmental factors in CLL development, which might partly explain the different incidence of CLL between China and Western countries.
