Gamma-aminobutyric acid B receptor regulates the expression of hydrogen sulfide/cystathionine-beta-synthase system in recurrent febrile seizures.
- Author:
Ying HAN
1
;
Jiong QIN
;
Ding-Fang BU
;
Xing-Zhi CHANG
;
Zhi-Xian YANG
;
Jun-Bao DU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Baclofen; pharmacology; Cystathionine beta-Synthase; genetics; physiology; Hydrogen Sulfide; blood; metabolism; Rats; Rats, Sprague-Dawley; Receptors, GABA-B; physiology; Recurrence; Seizures, Febrile; metabolism
- From: Chinese Journal of Contemporary Pediatrics 2006;8(2):141-143
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEFebrile seizure (FS) is one of the most common seizure types in children. Our previous studies have demonstrated that both gamma-aminobutyric acid B receptor (GABABR) and hydrogen sulfide (H2S) are involved in the pathogenesis of FS. This study was designed to explore the effect of GABABR on H2S/cystathionine-beta-synthase (CBS) system in recurrent FS.
METHODSSixty-four Sprague-Dawley rats aged 21 days were randomly assigned into four groups: Control (37 degrees C water bath exposure), FS, FS+baclofen (GABABR excitomotor), and FS+phaclofen (GABABR inhibitor) groups (n=16 each). FS was induced by warm water bath exposure (45.2 degrees C, once every 2 days, 10 times in total. The plasma level of H2S was detected by the spectrophotometer. The expression of CBS mRNA was examined by in situ hybridization. The expressions of CBS protein was observed by immunohistochemistry.
RESULTSThe plasma level of H2S increased in the FS+baclofen group (427.45 +/- 15.91 micromol/L) but decreased in the FS+phaclofen group (189.72 +/- 21.53 micromol/L) compared with that in the FS group (362.14 +/- 19.71 micromol/L). The expressions of CBS mRNA and protein were up-regulated in the FS+baclofen group but were down-regulated in the FS+phaclofen group compared with those in the FS group.
CONCLUSIONSGABABR modulated the expression of H2S/CBS system in recurrent FS.
