Transdifferentiation and collagen-synthesis effects of exogenous connective tissue growth factor on renal tubular epithelial cells in vitro.
- Author:
Ming QIAN
1
;
Wei-Hua GAN
;
Rong-Hua CHEN
;
Xiao-Qin PAN
;
Li FEI
Author Information
- Publication Type:Journal Article
- MeSH: Actins; genetics; Cadherins; genetics; Cell Differentiation; drug effects; Cell Line; Collagen; biosynthesis; genetics; Connective Tissue Growth Factor; Epithelial Cells; cytology; drug effects; Humans; Immediate-Early Proteins; pharmacology; Intercellular Signaling Peptides and Proteins; pharmacology; Kidney Tubules; cytology; drug effects; metabolism; RNA, Messenger; analysis; Recombinant Proteins; pharmacology
- From: Chinese Journal of Contemporary Pediatrics 2006;8(2):144-146
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of recombinal human connective tissue growth factor (rhCTGF) stimulation on epithelial-myofibroblast transdifferentiation (EMT) and collagen-synthesis in human renal tubular epithelial cell line (HK2) in vitro.
METHODSThe cultured HK2 cells were stimulated with rhCTGF of 5 ng/mL. The morphological changes were observed under an inverted microscope. The cells were collected at 0, 3, 6, 12, 24 and 48 hrs after rhCTGF stimulation. The expression of E-cadherin,alpha-smooth muscle actin (alpha-SMA), collagen Ialpha1 (Col Ialpha1) and collagen IValpha1 (Col IValpha1) mRNAs were detected by RT-PCR.
RESULTSrhCTGF stimulation changed the HK2 cell appearance from oval to fusiformdown-regulated the E-cadherin mRNA expression and up-regulated alpha-SMA mRNA expression, but had no effects the Col Ialpha1 and Col IValpha1 mRNA expression.
CONCLUSIONSExogenous CTGF can mediate the EMT but has no collagen-synthesis effects on HK2 cells.
