Asparagine synthetase activity in pediatric acute lymphoblastic leukemia.
- Author:
Hua JIANG
1
;
Long-Jun GU
;
Hui-Liang XUE
;
Jing-Yan TANG
;
Jing CHEN
;
Ci PAN
;
Jing CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Asparaginase; therapeutic use; Aspartate-Ammonia Ligase; genetics; metabolism; Child; Core Binding Factor Alpha 2 Subunit; genetics; Female; Humans; Male; Oncogene Proteins, Fusion; genetics; Precursor Cell Lymphoblastic Leukemia-Lymphoma; drug therapy; enzymology; RNA, Messenger; analysis
- From: Chinese Journal of Contemporary Pediatrics 2006;8(4):272-274
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the cellular activity of asparagine synthetase in different types of childhood acute lymphoblastic leukemia (ALL).
METHODSThe cellular activity of asparagine synthetase was detected by HPLC-FLD and Protein measurement in 28 ALL children (7 cases of T-ALL and 21 cases of B-lymphoid lineage ALL) before chemotherapy.
RESULTSThe asparagines synthetase activity levels in T-ALL children were significantly higher than those of the B-lymphoid lineage ALL patients, with the median activity level of 9.3 nM Asn/mg protein/hr vs 5.2 nM Asn/mg protein/hr (P < 0.05). The distribution of the asparagine synthetase activity demonstrated a polymorphism in either T-ALL or B-lymphoid lineage ALL patients.
CONCLUSIONSThe cellular activity of asparagines synthetase in ALL patients is presented with a polymorphism distribution. The asparagines synthetase activity levels in T-ALL are significantly higher than in B-lymphoid lineage ALL.
