Anticancer effect of total annonaceous acetogenins on hepatocarcinoma.

Run-mei Yang; Wen-min LI; Wei-jun HU; Wen-hua Huang; Chun-yan Zhu; Jing-guang YU; Xin Zhao; Da-yong Cai; Nan-nan Gao

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Anticancer effect of total annonaceous acetogenins on hepatocarcinoma.

Author: Run-mei YANG ¹ ; Wen-min LI ¹ ; Wei-jun HU ² ; Wen-hua HUANG ¹ ; Chun-yan ZHU ¹ ; Jing-guang YU ¹ ; Xin ZHAO ¹ ; Da-yong CAI ¹ ; Nan-nan GAO ³
Author Information

1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, 100193, China.
2. Henan Institute of Engineering, Zhengzhou, 451191, China.
3. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, 100193, China. gaonann@126.com.
Publication Type:Journal Article
Keywords: annonaceous acetogenins; apoptosis; hepatocarcinoma; proliferation
MeSH: Acetogenins; chemistry; pharmacology; therapeutic use; Animals; Annona; chemistry; Antineoplastic Agents, Phytogenic; chemistry; pharmacology; therapeutic use; Apoptosis; drug effects; Carcinoma, Hepatocellular; drug therapy; enzymology; pathology; Caspases; metabolism; Cell Cycle; drug effects; Cell Line, Tumor; Cell Proliferation; drug effects; Chromatography, High Pressure Liquid; Dose-Response Relationship, Drug; Humans; Liver Neoplasms; drug therapy; enzymology; pathology; Male; Membrane Potential, Mitochondrial; drug effects; Mice; Organ Specificity; drug effects; Spleen; drug effects; Thymus Gland; drug effects; Xenograft Model Antitumor Assays
From: Chinese journal of integrative medicine 2015;21(9):682-688
CountryChina
Language:English
Abstract:
OBJECTIVETo confirm the anticancer effect of total annonaceous acetogenins (TAAs) abstracted from Annona squamosa Linn. on human hepatocarcinoma.
METHODSThe inhibitory effect of TAAs was demonstrated in H22-bearing mice. The potency of TAAs was confirmed as its 50% inhibiting concentration (IC50) on Bel-7402 cell under Sulfur Rhodamine B staining. Both underlying mechanisms were explored as cellular apoptosis and cell cycle under flow cytometry. Mitochondrial and recipient apoptotic pathways were differentiated as mitochondrial membrane potential under flow cytometry and caspases activities under fluorescence analysis.
RESULTSThe inhibitory rate of TAAs in mice was 50.98% at 4 mg/kg dose. The IC50 of TAAs on Bel-7402 was 20.06 µg/mL (15.13-26.61µg/mL). Effective mechanisms of TAAs were confirmed as both of arresting cell cycle at G1 phase and inducing apoptosis dose- and time-dependently. Mitochondrial and recipient pathways involved in apoptotic actions of TAAs.
CONCLUSIONTAAs is effective for hepatocarcinoma, via inhibiting proliferation and inducing apoptosis.