Role of microRNA-181a in the apoptosis of tubular epithelial cell induced by cisplatin.
- Author:
Han-Yu ZHU
1
;
Mo-Yan LIU
;
Quan HONG
;
Dong ZHANG
;
Wen-Jia GENG
;
Yuan-Sheng XIE
;
Xiang-Mei CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; genetics; Blotting, Western; Cell Line; Cisplatin; pharmacology; Flow Cytometry; Humans; MicroRNAs; genetics; metabolism; Proto-Oncogene Proteins c-bcl-2; metabolism; RNA, Small Interfering; genetics; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; Transfection; bcl-2-Associated X Protein; metabolism
- From: Chinese Medical Journal 2012;125(3):523-526
- CountryChina
- Language:English
-
Abstract:
BACKGROUNDCisplatin (DDP) is one of most effective and most commonly used therapeutic agent in treating tumors, it can accumulate in the kidney and lead to acute renal failure. MicroRNA-181a can induce cell apoptosis by suppressing the expression of Bcl-2 family. In the present study, we investigated the role of microRNA-181a in the apoptosis of tubular epithelial cell induced by DDP.
METHODSHK-2 cells were cultured, transfected with microRNA-181a inhibitor for 48 hours, and stimulated with 50 µmol/L cisplatin for 24 hours. MicroRNA-181a expression was analyzed by real time PCR, and cell apoptosis was detected by flow cytometry. Moreover, Bcl-2 and Bcl-2-associated X protein (Bax) expression were measured by Western blotting.
RESULTSMicroRNA-181a expression significantly down-regulated in cells transfected with microRNA-181a inhibitor, compared with that in untransfectd cells (21.19 ± 2.01 vs. 38.87 ± 1.97, P < 0.05). Cell apoptosis induced by DDP significantly decreased in cells transfected with MicroRNA-181a inhibitor. Compared with DDP treated cells alone, Bcl-2 expression strikingly was up-regulated and Bax expression was down-regulated in cells transfected with microRNA-181a inhibitor.
CONCLUSIONOne pathway of DDP induces apoptosis of tubular epithelial cell by suppressing Bcl-2 expression is achieved by regulating the target gene of MicroRNA-181a.
