Antitumour efficacy of a novel oncolytic adenovirus Ad-TD-RFP for human nasopharyngeal carcinoma
10.3760/cma.j.issn.1673-0860.2012.08.013
- VernacularTitle:新型溶肿瘤腺病毒Ad-TD-RFP对人鼻咽癌细胞的治疗作用
- Author:
Hua CAO
1
;
Kun XU
;
Peng-Ju WANG
;
Guo-Zhong JIANG
;
Dong-Ling GAO
;
Ji-Wei WANG
;
Feng-Yu CAO
;
Yao-He WANG
Author Information
1. 郑州大学附属第一医院
- Keywords:
Nasopharyngeal neoplasms;
Adenoviridae;
Oncolytic virotherapy;
Cell line,tumor
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2012;47(8):672-676
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluat the antitumor efficacy of Ad-TD-RFP for human nasopharyngeal carcinoma cells ( C666-1 ) in vitro and in vivo.Methods The oncolytic effects of Ad-TD-RFP and control virus dl11520 on C666-1 cells were determined by cytotoxicity assay (MTS assay).Viral replication of AdTD-RFP and dl11520 was detected at different time points (24 h,48 h,72 h and 96 h) by tissue culture infective dose (TCID50) in C666-1 cells implanted subcutaneously into the flank in each of BALB/c nude mice.The xenografts were injected intratumorally with Ad-TD-RFP or dl1520 to investigate their effects on tumor growth.Results The concentration for 50％ of maximal effect ( EC50 ) values of Ad-TD-RFP and dl1520 were ( 107.6 ±3.2) pt/cell and ( 174.1 ±4.0) pt/cell,respectively (t =22.6,P ＜0.001 ).The Ad-TD-RFP replication was 3 - 14 folds more than dl1520 replication at four time points (24 h,48 h,72 h and 96 h) in C666-1 cells (t values were 33.6,23.4,20.8 and 17.3,respectively,P＜0.001).The average tumor volumes of PBS group,dl1520 group and Ad-TD-RFP group were ( 1765.5 ± 713.9 ) mm3,( 1036.9 ± 623.8 ) mm3,and (420.8 ± 238.7 )mm3,respectively ( F =1 2.0,P ＜ 0.05 ) on day 67 after treatment.Conclusions The antitumour efficacy of the novel oncolytic adenovirus Ad-TD-RFP for human nasopharyngeal carcinoma C666-1 cells is superior to that of dl1520 in vitro and in vivo.The outcome of this study provides an experimental basis for the tratment of human nasopharyngeal carcinoma by viral gere therapy.
