Expression of metallothionein 2 in middle ear cholesteatoma and it's correlation with interleukin 1α and interleukin 6
10.3760/cma.j.issn.1673-0860.2012.10.009
- VernacularTitle:金属硫蛋白2及白细胞介素1α和6在中耳胆脂瘤组织中的表达及意义
- Author:
Yang LIU
1
;
Lin-Lin YU
;
Jun HAN
;
Jing-Ning CHENG
;
Da-Zhang YANG
Author Information
1. 卫生部中日友好医院
- Keywords:
Cholesteatoma,middle ear;
Metallothionein;
Interleukin-1 alpha;
Interleukin-6;
Cell line
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2012;47(10):831-835
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the expression and distribution of metallothionein 2 (MT-2) in middle ear cholesteatoma,and to explore the relationship of MT-2 with interleukin 1α (IL-1α) and interleukin 6 (IL-6),and well as to explore the role of MT-2 in the pathogenetic mechanism of middle ear cholesteatoma.Methods Using the immunohistochemistry and reverse transcription polymerase chain reaction to examine the expressions of MT-2,IL-1 α and IL-6 protein and MT-2 mRNA in twenty-five middle ears' cholesteatoma and seven normal external ears' canal skin.The influence of cholesteatoma debris on the MT-2 mRNA and protein of HaCaT cell were further analyzed.Results According to immunohistochemistry research,the expressions of MT-2,IL-1 α and IL-6 were extremely higher in middle ear cholesteatoma than those in normal external ears' canal skin(P ＜ 0.05 ).The result of RT-PCR showed that there was significant difference between the expression of MT-2 mRNA of and middle ear cholesteatoma than those in normal external ear canal skin( t =15.38,P ＜ 0.05 ).There was a positive correlation between the expression of MT-2 and that of IL-1 α( r =0.856,P ＜ 0.05).There was also positive correlation between the expression of MT-2 and that of IL-6 ( r =0.714,P ＜ 0.05 ). MT-2 mRNA and protein of HaCaT cell significantly increased when exposed to cholesteatoma debris in a dose dependent manner.Conclusions MT-2,IL-1 αand IL-6 may play an important role in the pathogenetic process of middle ear cholesteatoma.Cholesteatoma debris may involve in the proliferation of middle ear cholesteatoma by activation of MT-2.
