Detrimental effects of ouabain on cochlear spiral ganglion cells in rats
10.3760/cma.j.issn.1673-0860.2012.11.011
- VernacularTitle:哇巴因对大鼠耳蜗螺旋神经节细胞的损伤
- Author:
Juan QU
1
;
Hua HUANG
;
Jie WANG
;
Wen-Juan MI
;
Li QIAO
;
Jian-Hua QIU
Author Information
1. 第四军医大学西京医院耳鼻咽喉头颈外科
- Keywords:
Ouabain;
Spiral ganglion;
Hearing loss,sensorineural;
Rats
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2012;47(11):926-930
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the detrimental effects of ouabain,in vivo and in vitro on cochlear spiral ganglion cells (SGC).Methods Seventy-five male SD rats were randomly divided into five groups.In addition to the normal control group,rats in other four groups received 0.01,0.02,0.05 mmol/L of ouabain or saline through cochlear scala tympani drilling.Seven days after surgery,the hearing threshold was measured by distortion product otoacoustic emissions (DPOAE) and auditory brainstem response (ABR) in rats.In the in vitro study,SGC were isolated from theSD rats (E14) and treated with 1 × 10-8mmol/L of ouabain.The damaged of SGC were detected after ouabain treatment using immunohistochemistry,transmission electron microscope and scanning electron microscope in vitro.Results After administration of ouabain,DPOAE did not change significantly.No significant difference in the amplitude of DPOAE could be observed among all the groups(P ＞0.05).Compared with saline and normal control,ABR threshold significantly increased in the ouabain treated groups (P ＜ 0.05),which correlated well with the concentrations of ouabain.Electron microscopy showed that after treated with ouabain,SGC presented degenerative changes,including the collapse of organelle structures,the karyothcca dissolving,and the myelin sheath disintegrating.Conclusion Ouabain can damage SGC,either in the in vivo or in vitro conditions.
