RNA-binding protein PTB in spermatogenesis: Progress in studies.
- Author:
Xian-Ming DOU
1
;
Xian-Sheng ZHANG
1
Author Information
1. Department of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, China.
- Publication Type:Journal Article
- Keywords:
RNA-binding protein;
polypyrimidine tract-binding protein;
post-transcriptional regulation;
spermatogenesis
- MeSH:
Animals;
Atrophy;
Gene Expression Regulation;
physiology;
Heterogeneous-Nuclear Ribonucleoproteins;
metabolism;
physiology;
Homeostasis;
Isoenzymes;
metabolism;
Male;
Mice;
Nerve Tissue Proteins;
metabolism;
physiology;
Phosphoglycerate Kinase;
metabolism;
Polypyrimidine Tract-Binding Protein;
metabolism;
physiology;
RNA, Messenger;
metabolism;
RNA-Binding Proteins;
Seminiferous Tubules;
pathology;
Spermatids;
metabolism;
Spermatocytes;
metabolism;
Spermatogenesis;
physiology;
Spermatogonia;
metabolism;
Testis;
metabolism
- From:
National Journal of Andrology
2016;22(9):856-860
- CountryChina
- Language:Chinese
-
Abstract:
RNA binding proteins (RBPs) regulate the function of cells by interacting with nascent transcripts and therefore are receiving increasing attention from researchers for their roles in tissue development and homeostasis. The polypyrimidine tract binding (PTB) protein family of RBPs are important posttranscriptional regulators of gene expression. Further investigations on the post-transcriptional regulation mechanisms and isoforms of PTB proteins in the spermatogenesis show that PTB protein 1 (Ptbp1) is a predominant isoform in mitotic cells (spermatogonia), while Ptbp2 predominates in meiotic spermatocytes and postmeiotic spermatids and binds to the specific 3' untranslated region (3' UTR) of the phosphoglycerate kinase 2 (Pgk-2) mRNA, which helps to stabilize Pgk-2 mRNA in male mouse germ cells. In case of Ptbp2 inactivation in the testis, the differentiation of germ cells arrests in the stage of round spermatids, with proliferation of multinucleated cells in the seminiferous tubule, increased apoptosis of spermatocytes, atrophy of seminiferous tubules, and lack of elongating spermatids, which consequently affects male fertility. This article presents an overview on the structure of the PTB protein and its role in regulating mammalian spermatogenesis.
