Modulation of activities and mRNA expression of cytochrome P450 isoenzymes in rat liver by Sophora flaveacens and coadministration with Veratrum nigrum.
- Author:
Jianming ZHOU
1
;
Yuguang WANG
;
Zhiwu CHEN
;
Yue GAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cytochrome P-450 Enzyme System; genetics; metabolism; Female; Gene Expression Regulation, Enzymologic; drug effects; Liver; drug effects; enzymology; Male; Plant Extracts; administration & dosage; RNA, Messenger; genetics; metabolism; Random Allocation; Rats; Rats, Wistar; Sophora; chemistry; Veratrum; chemistry
- From: China Journal of Chinese Materia Medica 2010;35(14):1845-1849
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the modulatory effect of Sophora flaveacens and co-administration with Veratrum nigrum on the activity and mRNA expression of cytochrome P450 isoenzymes in rat liver.
METHODRat liver microsomal cytochrome P450, b5, aminopyrine N-demethylase (APND), p-nitrophenol-hydroxylase (pNPH) activities were quantitated by UV chromatography. The mRNA expression level of five CYP isoenzymes CYP1A1, CYP2B1/2, CYP2C11, CYP2E1 and CYP3A1 were detected by semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR).
RESULTS. flaveacen and its combination with V. nigrum can dramatically reduce P450 and b5 protein content. Both single and combined use inhibited the activities of aminopyrine N-demethylase. At the mRNA level, the expression of CYP2C11 markedly induced exposure to V. nigrum, while decreased after combination with S. flaveacens. S. flaveacens can induce CYP2B1/2 gene expression, while the Sophora- Veratrum group showed weak inhibition. Both single and co-administrated group have some inhibitory effect on CYP3A1 gene expression, while CYP1A gene expression almost has no change in each group.
CONCLUSIONThese results indicated that compatibility of Sophora and Veratrum in a prescription also have herb-herb interactions based on P450. The possible mechanisms of its incompatible effects need to be further analysised via integrating with metabolic studies.
