Effects of resveratrol on proliferation and apoptosis of TNF-alpha induced rheumatoid arthritis fibroblast-like synoviocytes.
- Author:
Jing TIAN
1
;
Jiesheng GAO
;
Jinwei CHEN
;
Fen LI
;
Xi XIE
;
Jinfeng DU
;
Jing WANG
;
Ni MAO
Author Information
- Publication Type:Journal Article
- MeSH: Aged; Apoptosis; drug effects; Arthritis, Rheumatoid; drug therapy; immunology; physiopathology; Cell Cycle; drug effects; Cell Proliferation; drug effects; Cells, Cultured; Female; Fibroblasts; cytology; drug effects; Humans; Male; Middle Aged; Stilbenes; pharmacology; Synovial Membrane; cytology; drug effects; immunology; Tumor Necrosis Factor-alpha; immunology
- From: China Journal of Chinese Materia Medica 2010;35(14):1878-1882
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of resveratrol (Res) on in vitro proliferation and apoptosis of TNF-alpha induced rheumatoid arthritis fibroblast-like synoviocytes (RA FLS), and further to investigate the PI3 K/Akt/BAD signal mechanism.
METHODThe inhibition rate of RA FLS was examined by MTT assay. Cell cycle and the amount of apoptotic cells was measured by flow cytometry. PI3K/Akt/BAD signal transduction proteins expression was measured by western blot.
RESULTThe living cells measured by MTT dose and time-dependently reduced in Res groups. In Res groups, the fraction of living cells in the S-phase and G2/M-phase decreased respectively, while that in G1-phase increased, the difference was statistically significant compared with the TNF-alpha group (P < 0.05). Flow cytometry demonstrated that the apoptosis rate increased with increased Res concentration. Res inhibited TNF-alpha induced phosphorylation of Akt and BAD in RA FLS.
CONCLUSIONRes can inhibit RA FLS proliferation and induce apoptosis through inhibition of PI3K/Akt/BAD signalling pathway. Res may provide a new therapeutic approach in treatment of RA.
